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RNAi載體,RNAi-Ready pSIREN-Retro Q-ZsGreen載體
| 型號 | 載體名稱 | 出品公司 | 載體用途 |
| VRC0347 | RNAi-Ready pSIREN-Retro Q-ZsGreen | Clontech | RNAi載體 |
Promoter: Human U6
Reporter: ZsGreen
Selection: prokaryotic—ampicillin
Replication: prokaryotic—Col E1 ori （low copy）, viral—SV40 ori
RNAi-Ready pSIREN-RetroQ-ZsGreen derives from our original gene silencing retroviral vector RNAi-Ready pSIREN-RetroQ. pSIREN-RetroQ-ZsGreen is a self-inactivating retroviral expression vector designed to express a small hairpin RNA （shRNA） using the human U6 promoter. This vector is provided as a linearized vector digested with BamH I and EcoR I. It is used for targeted gene silencing when an annealed DNA oligonucleotide encoding an appropriate shRNA is ligated into the vector. The pSIREN-RetroQ-ZsGreen construct can be transfected as a plasmid expression vector. For generation of active retrovirus for infecting hard-to-transfect cell lines, transfect the pSIREN-RetroQ-ZsGreen construct into a retroviral packaging cell line and collect the viral supernatant.
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